GenCRISPR™ Cas9 v1.2 is a tag free nuclease produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes with a biparticle nucleus localization signal (BPNLS) at N-terminal and a nucleoplasmin nucleus localization signal (nucleoplasmin NLS) at C-terminal. It has been reported that BPNLS and nucleoplasmin NLS are able to improve the gene editing efficiency.

Description

The GenCRISPR™ Cas9 v1.2 can be formed with the guide RNA into a ribonucleoprotien (RNP) complex. The use of an RNP complex to perform gene editing has been shown to reduce the challenges encountered with other CRISPR gene editing techniques such as viral and plasmid delivery. Challenges include off-target effects, cell viability and transcription/translational challenges.GenCRISPR™ Cas9 v1.2 is a tag free nuclease produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes with a biparticle nucleus localization signal (BPNLS) at N-terminal and a nucleoplasmin nucleus localization signal (nucleoplasmin NLS) at C-terminal. It has been reported that BPNLS and nucleoplasmin NLS are able to improve the gene editing efficiency.  

Note

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

產(chǎn)品概述

Key Features 

High gene editing efficiencies: Consistent high editing efficiency in in-vitro and in-vivo.

Tag-free: Amino acid is free from additional tagging amino acid. 

DNA-free: No external DNA added to the system.

應用指導 

gRNA-dependent double-stranded DNA cleavage

產(chǎn)品屬性

Source :Recombinant Cas9 with a BPNLS at N-terminal and a nucleoplasmin NLS at C-terminal expressed by E.coli 

Species :Streptococcus pyogenes

Tag :Tag-free

Molecular Weight :~160 kDa

Concentration :4 mg/ml

Active temperature :This Cas9 is active at 37°C.

Formulation :Supplied as a solution of 25 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 50% glycerol, pH 8.0.

Storage & Stability :This product remains stable for up to 12 months at -20°C. Avoid repeated freeze-thaw cycles. 

質控參數(shù)

Appearance: Clear, colorless liquid

Purity :≥ 90% as analyzed by SDS-PAGE

Concentration :4 mg/ml±10% 

Bioactivity: ≥ 90%

Residual DNase: Non-specific DNase activity

Residual RNase: Non-specific RNase activity

Endotoxin Level :≤ 100 EU/mg as analyzed by gel clotting method